Calreticulin Recombinant?Rabbit?Monoclonal Antibody?Product?Datasheet Catalog# ?BX50302?Clone# ?BP6279 Predicted?Molecular Wt: 48kDa Purity: ??ProA?affinity?purified?IgG Species Cross-reactivity: Human Form: ??Liquid Applications: IHC-P Swissprot?ID: ??P27797 Background: Calcium?is a?universal signaling?molecule?involved?in?many cellular functions, such?as?cell?motility,?metabolism,?protein modification, et al.?Calcium?is stored?in the endoplasmic?reticulum?(ER),?where?it?is?buffered?by?calcium?binding?chaperones?such as calnexin and calreticulin.?Calreticulin?also functions?asan?ER?chaperone?that?ensures?proper?folding?and?quality control of?newly synthesized glycoproteins. Calreticulin?is expressed?in all?kinds of?normal tissues and?tumor?tissues. Calreticulin?purified from tumor can stimulate the anti-tumor?immune?response?of the?body,?the?possible?mechanism?is?that???the calreticulin?molecule?binds to the tumor?antigen?polypeptide, and?in the?process?of?calreticulinto?the?cell?membrane,?the?tumor antigen?peptide?bound with?it?is?brought to the cell?membrane and?presented?to?the?antigen-presenting?cell,?thus stimulating the anti-tumor?immune effect of the?body. Calreticulin?purified?from?tumor?can?stimulate?the?anti-tumor?immune??response of the?body, the?possible?mechanism?is that the calreticulin?molecule?binds to the tumor?antigen?polypeptide,?and?in?the?process of calreticulinto the cell?membrane, the tumor?antigen?peptide?bound with?it?is?brought?to?the?cell?membrane and?presented to the antigen-presenting cell, thus stimulating the?anti-tumor?immune?effect?of the?body. Subcellular?location: Cytoplasm Recommended?Method: Heat?induced epitope?retrieval with Tris-EDTA?buffer???(pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)?for?30?minutes. Immunogen: Synthetic?peptide. This?information?is?proprietary to?Biolynx. Storage?Buffer: PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA 0.05%. Storage Conditions: -25°C?to?-18°C Shipment?Instructions: Shipped?on?blue?ice.?Upon?delivery?store?at?-25°C?to?–?18°C. Avoid?freeze?/ thaw?cycles. Recommended?Dilution: IHC-P:?????1:100-1:200 Background?References: 1.?Groenendyk, J. et?al.?(2004)?Mol?Cells?17,?383-9. 2.?Williams,?D.B.?(2006) J?Cell?Sci?119,?615-23. Immunohistochemistry?(Formalin/PFA-fixed?paraffin-embedded?sections)?analysis of ?human?prostate?labelling Calreticulin?with???BP6279.
杭州浩克生物
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