PCNA鼠單克隆抗體

品牌	商品編碼	商品名稱	規(guī)格	單位	單價
HAOKEBIO	HK510145L	PCNA鼠單克隆抗體	200ul	支	2800

Applications

Tested Applications:

FC, IF, IHC, IP, WB, ELISA

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Cited Applications:

IF, IHC, WB

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Species Specificity:

human, mouse, pig, rat

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Cited Species:

human, mouse, pig, rabbit, rat

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Positive WB detected in:

HEK-293 cells, HeLa cells, HepG2 cells

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Positive IP detected in:

HepG2 cells

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Positive IHC detected in:

human gliomas tissue, human tonsillitis tissue, human breast cancer tissue, mouse ovary tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

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Positive IF detected in:

HepG2 cells, mouse heart tissue

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Positive FC detected in:

MCF-7 cells

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Recommended dilution:

WB : 1:5000-1:50000

IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB

IHC : 1:500-1:2000

IF : 1:250-1:1000

Product Information

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Source:

Mouse

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Purification method:

Protein A purification

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Isotype:

IgG1

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Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

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Full name:

proliferating cell nuclear antigen

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Calculated molecular weight:

29 kDa/31 kDa

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Observed molecular weight:

36-38 kDa

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Gene symbol

PCNA

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Synonyms

Cyclin, PCNA

Background

Proliferating Cell Nuclear Antigen, commonly known as PCNA, is a protein that acts as a processivity factor for DNA polymerase δ in eukaryotic cells. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase’s processibility during elongation of the leading strand. PCNA induces a robust stimulatory effect on the 3′-5′ exonuclease and 3′-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. It has to be loaded onto DNA in order to be able to stimulate APEX2. PCNA protein is highly conserved during evolution; the deduced amino acid sequences of rat and human differ by only 4 of 261 amino acids. PCNA has been used as loading control for proliferating cells. The calculated molecular weight of PCNA is 29 kDa, but modified PCNA is 36kDa (PMID: 1358458).