Cited Applications: ICC/IF, IF, WB Species Specificity: human, mouse, rat
Cited Species: human, mouse
Positive WB detected in: HT-29 cells, A549 cells, rat testis tissue, mouse testis tissue, HeLa cells, Jurkat cells, SW480 cells
Positive IP detected in: mouse brain tissue, NIH/3T3 cells
Positive IHC detected in: human breast cancer tissue Note: suggested antigen retrieval with?TE buffer pH 9.0;?(*) Alternatively, antigen retrieval may be performed with?citrate buffer pH 6.0
Positive IF detected in: HepG2 cells
Positive FC detected in: HepG2 cells
Recommended dilution: WB : 1:2000-1:10000 IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB IHC : 1:1000-1:4000 IF : 1:10-1:100 Product Information
Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20°C. Stable for one year after shipment.?Aliquoting is unnecessary for -20oC storage. Immunogen Information
Full name: heat shock 70kDa protein 9 (mortalin)
Calculated molecular weight: 74 kDa
Observed molecular weight: 74-75 kDa
Gene symbol HSPA9
Synonyms CSA, GRP 75, GRP75, Heat shock 70 kDa protein 9, HSPA9, HSPA9B, Mortalin, MOT, mot 2, MOT2, MTHSP75, PBP74, Peptide binding protein 74 Background GRP75 (also known as mortalin, HSPA9 or mt-Hsp70) is a constitutively expressed member of the HSPA (HSP70) family of heat-shock proteins. It is located in the mitochondrial matrix and anti-GRP75 is commonly used as the marker for mitochondria. It has been reported that GRP75 is enriched in cancer cells and contributes to carcinogenesis. In addition, decreased expression level of GRP75 has been found in neurodegenerative disorders like Parkinson’s disease. This antibody recognized the endogenous GRP75 protein in NIH/3T3 cells. (PMID: 21640711, 22920904)
杭州浩克生物
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